anti hck Search Results


hck antibody n 30  (Santa Cruz Biotechnology)
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hck  (Bio-Rad)
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Bio-Rad hck
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hck 394903  (R&D Systems)
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mouse monoclonal anti human hck antibody  (Proteintech)
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Proteintech mouse monoclonal anti human hck antibody
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anti-hck monoclonal antibodies 18/hck  (Becton Dickinson)
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Anti Hck Monoclonal Antibodies 18/Hck, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-rabbit hck antibody a14537  (ABclonal Biotechnology)
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hck  (Becton Dickinson)
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Becton Dickinson hck
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monoclonal anti-human-hck  (Becton Dickinson)
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mouse anti-hck  (Becton Dickinson)
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Becton Dickinson mouse anti-hck
A ) Confocal IFA of intracellular localization of MAP4K4 in parasitized (upper) and BW720c-cured (lower) TaC12 cells. Red: actin, green: MAP4K4, blue: TaSP. Right: magnification of TaC12 lamellipodium showing accumulation of MAP4K4 at leading edge. Schema shows localization of MAP4K4 at leading edge of cell. B ) IB analysis of untreated and BW720c-treated TaC12 cells using <t>anti-MAP4K4,</t> <t>anti-ERM,</t> <t>anti-Hck</t> and anti-tubulin antibodies. Bar diagram below shows mean protein abundances −/+ SDs. in control and BW720c-treated TaC12 cells. C ) In vitro kinase assay comparing MAP4K4 immunoprecipitated either from infected or cured TaC12 cells. Upper: IB of immunoprecipitated MAP4K4, lower: autorad of total protein phosphorylation activities immunoprecipitated. D ) Upper: IB analysis with anti-MAP4K4 and anti-tubulin antibodies using lysates of TaC12 cells previously incubated with increasing concentrations of conditioned medium. Lower: Mean integrated densities of MAP4K4 bands (−/+ SDs, normalized to tubulin) E ) Quantification of MAP4K4 depletion by qRT-PCR using primers p81 and p79 (upper, see also ) or IB (lower) 24 h after siControl or siMAP4K4 transfection. F ) 12 h single cell migration analysis of cells transfected with either siControl or siMAP4K4_1 or siMAP4K4_2. Box plot show means of path lengths (n = 34, 38, 44). G ) Matrigel invasion assay: Bright field microscopy images (100× magnification) of transmigrated siControl or siMAP4K4_1 or siMAP4K4_2 transfected cells after 24 h in the presence of 25 ng/ml TNFα. H ) Quantification of images shown in G expressed as relative transmigration compared to siControl and SDs. See also and .
Mouse Anti Hck, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-human hck monoclonal antibody  (Becton Dickinson)
90
Becton Dickinson mouse anti-human hck monoclonal antibody
A ) Confocal IFA of intracellular localization of MAP4K4 in parasitized (upper) and BW720c-cured (lower) TaC12 cells. Red: actin, green: MAP4K4, blue: TaSP. Right: magnification of TaC12 lamellipodium showing accumulation of MAP4K4 at leading edge. Schema shows localization of MAP4K4 at leading edge of cell. B ) IB analysis of untreated and BW720c-treated TaC12 cells using <t>anti-MAP4K4,</t> <t>anti-ERM,</t> <t>anti-Hck</t> and anti-tubulin antibodies. Bar diagram below shows mean protein abundances −/+ SDs. in control and BW720c-treated TaC12 cells. C ) In vitro kinase assay comparing MAP4K4 immunoprecipitated either from infected or cured TaC12 cells. Upper: IB of immunoprecipitated MAP4K4, lower: autorad of total protein phosphorylation activities immunoprecipitated. D ) Upper: IB analysis with anti-MAP4K4 and anti-tubulin antibodies using lysates of TaC12 cells previously incubated with increasing concentrations of conditioned medium. Lower: Mean integrated densities of MAP4K4 bands (−/+ SDs, normalized to tubulin) E ) Quantification of MAP4K4 depletion by qRT-PCR using primers p81 and p79 (upper, see also ) or IB (lower) 24 h after siControl or siMAP4K4 transfection. F ) 12 h single cell migration analysis of cells transfected with either siControl or siMAP4K4_1 or siMAP4K4_2. Box plot show means of path lengths (n = 34, 38, 44). G ) Matrigel invasion assay: Bright field microscopy images (100× magnification) of transmigrated siControl or siMAP4K4_1 or siMAP4K4_2 transfected cells after 24 h in the presence of 25 ng/ml TNFα. H ) Quantification of images shown in G expressed as relative transmigration compared to siControl and SDs. See also and .
Mouse Anti Human Hck Monoclonal Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-human hck monoclonal antibody - by Bioz Stars, 2026-07
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anti-hck mab  (Becton Dickinson)
90
Becton Dickinson anti-hck mab
A ) Confocal IFA of intracellular localization of MAP4K4 in parasitized (upper) and BW720c-cured (lower) TaC12 cells. Red: actin, green: MAP4K4, blue: TaSP. Right: magnification of TaC12 lamellipodium showing accumulation of MAP4K4 at leading edge. Schema shows localization of MAP4K4 at leading edge of cell. B ) IB analysis of untreated and BW720c-treated TaC12 cells using <t>anti-MAP4K4,</t> <t>anti-ERM,</t> <t>anti-Hck</t> and anti-tubulin antibodies. Bar diagram below shows mean protein abundances −/+ SDs. in control and BW720c-treated TaC12 cells. C ) In vitro kinase assay comparing MAP4K4 immunoprecipitated either from infected or cured TaC12 cells. Upper: IB of immunoprecipitated MAP4K4, lower: autorad of total protein phosphorylation activities immunoprecipitated. D ) Upper: IB analysis with anti-MAP4K4 and anti-tubulin antibodies using lysates of TaC12 cells previously incubated with increasing concentrations of conditioned medium. Lower: Mean integrated densities of MAP4K4 bands (−/+ SDs, normalized to tubulin) E ) Quantification of MAP4K4 depletion by qRT-PCR using primers p81 and p79 (upper, see also ) or IB (lower) 24 h after siControl or siMAP4K4 transfection. F ) 12 h single cell migration analysis of cells transfected with either siControl or siMAP4K4_1 or siMAP4K4_2. Box plot show means of path lengths (n = 34, 38, 44). G ) Matrigel invasion assay: Bright field microscopy images (100× magnification) of transmigrated siControl or siMAP4K4_1 or siMAP4K4_2 transfected cells after 24 h in the presence of 25 ng/ml TNFα. H ) Quantification of images shown in G expressed as relative transmigration compared to siControl and SDs. See also and .
Anti Hck Mab, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+hck/pm16443220-43-39-42?v=Becton+Dickinson
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anti hck antibody  (Upstate Biotechnology Inc)
86
Upstate Biotechnology Inc anti hck antibody
A ) Confocal IFA of intracellular localization of MAP4K4 in parasitized (upper) and BW720c-cured (lower) TaC12 cells. Red: actin, green: MAP4K4, blue: TaSP. Right: magnification of TaC12 lamellipodium showing accumulation of MAP4K4 at leading edge. Schema shows localization of MAP4K4 at leading edge of cell. B ) IB analysis of untreated and BW720c-treated TaC12 cells using <t>anti-MAP4K4,</t> <t>anti-ERM,</t> <t>anti-Hck</t> and anti-tubulin antibodies. Bar diagram below shows mean protein abundances −/+ SDs. in control and BW720c-treated TaC12 cells. C ) In vitro kinase assay comparing MAP4K4 immunoprecipitated either from infected or cured TaC12 cells. Upper: IB of immunoprecipitated MAP4K4, lower: autorad of total protein phosphorylation activities immunoprecipitated. D ) Upper: IB analysis with anti-MAP4K4 and anti-tubulin antibodies using lysates of TaC12 cells previously incubated with increasing concentrations of conditioned medium. Lower: Mean integrated densities of MAP4K4 bands (−/+ SDs, normalized to tubulin) E ) Quantification of MAP4K4 depletion by qRT-PCR using primers p81 and p79 (upper, see also ) or IB (lower) 24 h after siControl or siMAP4K4 transfection. F ) 12 h single cell migration analysis of cells transfected with either siControl or siMAP4K4_1 or siMAP4K4_2. Box plot show means of path lengths (n = 34, 38, 44). G ) Matrigel invasion assay: Bright field microscopy images (100× magnification) of transmigrated siControl or siMAP4K4_1 or siMAP4K4_2 transfected cells after 24 h in the presence of 25 ng/ml TNFα. H ) Quantification of images shown in G expressed as relative transmigration compared to siControl and SDs. See also and .
Anti Hck Antibody, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+hck/10__1074_slash_jbc__m402290200-110-117-121?v=Upstate+Biotechnology+Inc
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Image Search Results


A ) Confocal IFA of intracellular localization of MAP4K4 in parasitized (upper) and BW720c-cured (lower) TaC12 cells. Red: actin, green: MAP4K4, blue: TaSP. Right: magnification of TaC12 lamellipodium showing accumulation of MAP4K4 at leading edge. Schema shows localization of MAP4K4 at leading edge of cell. B ) IB analysis of untreated and BW720c-treated TaC12 cells using anti-MAP4K4, anti-ERM, anti-Hck and anti-tubulin antibodies. Bar diagram below shows mean protein abundances −/+ SDs. in control and BW720c-treated TaC12 cells. C ) In vitro kinase assay comparing MAP4K4 immunoprecipitated either from infected or cured TaC12 cells. Upper: IB of immunoprecipitated MAP4K4, lower: autorad of total protein phosphorylation activities immunoprecipitated. D ) Upper: IB analysis with anti-MAP4K4 and anti-tubulin antibodies using lysates of TaC12 cells previously incubated with increasing concentrations of conditioned medium. Lower: Mean integrated densities of MAP4K4 bands (−/+ SDs, normalized to tubulin) E ) Quantification of MAP4K4 depletion by qRT-PCR using primers p81 and p79 (upper, see also ) or IB (lower) 24 h after siControl or siMAP4K4 transfection. F ) 12 h single cell migration analysis of cells transfected with either siControl or siMAP4K4_1 or siMAP4K4_2. Box plot show means of path lengths (n = 34, 38, 44). G ) Matrigel invasion assay: Bright field microscopy images (100× magnification) of transmigrated siControl or siMAP4K4_1 or siMAP4K4_2 transfected cells after 24 h in the presence of 25 ng/ml TNFα. H ) Quantification of images shown in G expressed as relative transmigration compared to siControl and SDs. See also and .

Journal: PLoS Pathogens

Article Title: Intracellular Theileria annulata Promote Invasive Cell Motility through Kinase Regulation of the Host Actin Cytoskeleton

doi: 10.1371/journal.ppat.1004003

Figure Lengend Snippet: A ) Confocal IFA of intracellular localization of MAP4K4 in parasitized (upper) and BW720c-cured (lower) TaC12 cells. Red: actin, green: MAP4K4, blue: TaSP. Right: magnification of TaC12 lamellipodium showing accumulation of MAP4K4 at leading edge. Schema shows localization of MAP4K4 at leading edge of cell. B ) IB analysis of untreated and BW720c-treated TaC12 cells using anti-MAP4K4, anti-ERM, anti-Hck and anti-tubulin antibodies. Bar diagram below shows mean protein abundances −/+ SDs. in control and BW720c-treated TaC12 cells. C ) In vitro kinase assay comparing MAP4K4 immunoprecipitated either from infected or cured TaC12 cells. Upper: IB of immunoprecipitated MAP4K4, lower: autorad of total protein phosphorylation activities immunoprecipitated. D ) Upper: IB analysis with anti-MAP4K4 and anti-tubulin antibodies using lysates of TaC12 cells previously incubated with increasing concentrations of conditioned medium. Lower: Mean integrated densities of MAP4K4 bands (−/+ SDs, normalized to tubulin) E ) Quantification of MAP4K4 depletion by qRT-PCR using primers p81 and p79 (upper, see also ) or IB (lower) 24 h after siControl or siMAP4K4 transfection. F ) 12 h single cell migration analysis of cells transfected with either siControl or siMAP4K4_1 or siMAP4K4_2. Box plot show means of path lengths (n = 34, 38, 44). G ) Matrigel invasion assay: Bright field microscopy images (100× magnification) of transmigrated siControl or siMAP4K4_1 or siMAP4K4_2 transfected cells after 24 h in the presence of 25 ng/ml TNFα. H ) Quantification of images shown in G expressed as relative transmigration compared to siControl and SDs. See also and .

Article Snippet: The antibodies used for immunoblotting were: rabbit anti-MAP4K4 (A301-502A, BETHYL, 1∶1000), rabbit anti-ERM (3142, Cell Signaling, 1∶1000), rabbit anti-Phospho-ERM (3149, Cell Signaling, 1∶1000), mouse anti-HcK (610278, BD Biosciences, 1∶1000), mouse anti-beta-Tubulin (T4026, SIGMA-ALDRICH, 1∶10'000), rabbit anti-Phospho-SAPK/JNK (Thr183/Tyr185) (4668, Cell Signaling, 1∶1000), rabbit anti –SAPK/JNK (9258, Cell Signaling, 1∶1000), rabbit anti-Phospho-ATF2 (Thr71) (9221, Cell Signaling, 1∶1000), rabbit anti-ATF2 (9226, Cell Signaling, 1∶1000), secondary anti-mouse and anti-rabbit (7076 and 7074, Cell signaling, 1∶5000).

Techniques: In Vitro, Kinase Assay, Immunoprecipitation, Infection, Incubation, Quantitative RT-PCR, Transfection, Migration, Invasion Assay, Microscopy, Transmigration Assay